alpha300 Ri - 倒立型共焦点ラマン顕微鏡

alpha300 Ri – Inverted Confocal Raman Imaging

  • Experience Raman Imaging from a new angle.

    倒立型光学系でラマンイメージが取得できます。alpha300Ri は、倒立型光学系を採用し、WITec 標準機であるalpha300R共焦点ラマン顕微鏡の機能をそのまま継承しています。

    特に、ライフサイエンス、バイオ医薬、製薬、鉱物の分野において液体試料や大型試料を観察する場合、alpha300Riの倒立型光学系が威力を発揮します。

     

特長

      • 試料を非破壊、非接触、非染色で観察
      • 高い共焦点性で高分解能3次元観察
      • 光学回析限界まで達する高分解能観察
      • 観察場所を容易に特定し、高い位置再現性
      • 大型試料への対応
      • モジュール構成により簡単にアップグレード
      • 蛍光観察、微分干渉観察(DIC)、位相差観察の機能拡張

アプリケーション例

    • 真核細胞の蛍光顕微鏡像とラマンイメージの合成

      核はDAPIにより染色(青色)

    • Correlative Raman Fluourescence Microscopy: Fluorescence DAPI Staining & Raman Imaging
  • 3D Raman image of pressed banana pulp

    The alpha300 Ri combines the advantages of data acquisition from below with the established merits of 3D confocal Raman imaging which is a powerful and versatile technique that can chemically characterize samples nondestructively and without labeling or other specialized preparation. 

    The image shows a 3D reconstrucion of Raman image-stack acquisition of banana pulp.

    Scan range: 300µm x 200µm x 90µm
    Pixels (Spectra): 450x300x45

  • Pressed banana pulp sample: Starch grains (green) and cell wall components (red)

仕様

  • ラマン一般操作モード

    • Raman spectral imaging: Acquisition of complete hyperspectral Raman imaging data sets while continuously scanning.
    • Planar (x-y-direction) and depth scans (z-direction) with motorized sample positioning
    • Image stacks: 3D confocal Raman imaging 
    • Fast and slow time series
    • Single point Raman spectrum acquisition and depth profiling 
    • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
    • Confocal Fluorescence Microscopy 

    基本顕微鏡特長

    • Research grade inverted 
    • Raman spectral imaging: Acquisition of complete hyperspectral Raman imaging data sets while continuously scanning.
    • Planar (x-y-direction) and depth scans (z-direction) with motorized sample positioning
    • Image stacks: 3D confocal Raman imaging   
    • Single point Raman spectrum acquisition and depth profiling
    • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
    • Confocal Fluorescence Microscopy 
    • optical microscope with 6x objective turret
    • Video system: Video CCD camera and/or fluorescence camera 
    • LED white-light source for Köhler illumination 
    • Binocular
    • Condensor lens for up to 7 contrasts (e. g. brightfield, DIC, Phase contrast, NAMC, etc.)
    • Sample holder accommodating various standard sample formats (e. g. microscopy slides, Terasakiplates, 35/65 mm dishes, counting chambers)
    • Internal filter block revolver
    • Motorized sample positioning and scanning stage in x- and y-direction, large travel range: 110 mm x 70 mm
  • Raman Optional/Upgradable Operation modes

    • A broad range of lasers and wavelenghts (from UV to IR) eligible
    • Additional UHTS-spectrometers (UV, VIS, NIR)
    • Ultrafast Raman imaging (1300 spectra per second) optional available
    • Upgradable for time correlated single photon counting / fluorescence lifetime imaging (FLIM)
    • Autofocus

    Ultrahigh-throughput WITec UHTS Spectrometers

    • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
    • Fibre-coupled ultrahigh-throughput optical instruments (up to 70 % transmission)
    • Superior peak shape conservation

    Computer Interface:

    • WITec software for instrument and measurement control, data evaluation and processing

Extensions & Related Instruments

製品カタログ

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